Alphanumeric grid, colored manufacturing, and the labeling surface. Roche lightcycler 480 realtime pcr run setup instructions for rt2 profiler pcr arrays. In the us our pharmaceuticals are marketed by genentech. The only difference between this updated version and lightcycler software 4. Benefit from cuttingedge thermal block and optical technology that leads to excellent welltowell homogeneity and maximized detection. As the world leader in invitro diagnostics, we supply a wide range. South african companies nailed for using pirated software. The lightcycler 480 software allows the user to easily alternate between screens e. Used roche lightcycler 480 ii real time pcr system lab. In my opinion lc480 software is also more userfriendly. The lightcycler 96 system embodies roches expertise over the last decade in developing and providing highperformance qpcr systems that enable research breakthroughs. Pcr was performed using a lightcycler realtime pcr instrument roche. The ixo file extension is associated with the lightcycler software, a program used to work with lightcycler realtime pcr system device from roche diagnostics it seems that this file.
The lightcycler 480 from roche is available for quantitative realtime pcr analysis and it can perform methylation analysis and high resolution melting analysis. Roche molecular biochemicals lightcycler operators manual. Lightcycler 480 instrument ii roche applied science. Roche diagnostics lightcycler 480 plat 384 white lightcycler. Operation training and onsite assistance are provided. Roche product information related to the us market. Brand pcr plates for roche lightcycler 480, brandtech vwr. Other areas include viral infections, metabolic, central nervous system disorders and inflammatory diseases. I have a problem with the analysis in lightcycler 480 software. Service manual light cycler please note that this is only.
The roche lightcycler 480 realtime pcr system offers realtime pcr analysis of gene expression, genetic variation and melting curvebased mutation. Make sure the correct thermal block cycler 96well for f plate or 384well block format for g plate is in place. Due to its highly flexible selection of excitation and emission wavelengths, the lightcycler 480 realtime pcr system is compatible with most currently available dyes and assay formats, yet is also adaptable to new, future applications. Lightcycler 480 gene scanning software roche life science. The lightcycler480 software provides an intuitive, easytonavigate user interface for realtime pcr programming, data capture, and data analysis. Lightcycler 480 detection formats roche applied science. Rely on the highly adaptable lightcycler 480 system and related software, kits, disposables, reagents, and probes to perform gene expression analysis, snp. New file format for roche devices lightcycler 480 has been added. Comprehensive suite of tools for setup, run, and analysis of realtime pcr quantification and genotyping assays on the lightcycler 480 real.
Equipment in the immune monitoring lab is open to all investigators. The lightcycler 480 system is a highperformance, flexible throughput qpcr platform interchangeable 96 or 384well blocks that provides a platebased, highly adaptable and versatile realtime pcr system for gene expression analysis, snp genotyping, and mutation scanning via high resolution melting hrm. Quantification cycle values were extracted with the lightcycler 480 software version 1. We spend countless hours researching various file formats and. Conforms to the european directive for in vitro diagnostic medical devices 9879ec in combination with the lightcycler 2. I prepared the twostep cycling experiment with the following conditions. This ixo file type entry was marked as obsolete and no longer supported file format. Opening new horizons in the field of data analysis. Lightcycler 1536 real time pcr system roche diagnotic india. Roche lightcycler 480 ii real time pcr system for 96 well plates. The ixo file extension is associated with the lightcycler software, a program used to work with lightcycler realtime pcr system device from roche diagnostics. We spend countless hours researching various file formats and software that can open, convert, create or otherwise work with those files.
The software is compatible with the regulations for electronic records subpart b. Roche lightcycler 480 real time pcr the lab world group. Thermo scientific armadillo pcr, clear semiskirted, low profile, clear well plates are the ultimate in high performance pcr plastics. A novel, multiplex, realtime pcrbased approach for the. The user can also choose which samples to include in the display and. With common well matrixes and high or low profile designs that are compatible with thermal cyclers, the pcr plates facilitate rapid segment amplification when manual processing is not wanted. Please refer to the lightcycler 480 operation manual and the lightcycler realtime pcr systems application manual for detailed descriptions and additional information on multiple protocols. Lightcycler 480 multiwell plate 384, white 5 x 10 plates includes sealing foils catalog no. This study is written according to the minimum information for publication of. Precisely engineered for miniaturization and parallelization, this latest innovation reinforces roche applied science as a global expert in realtime pcr.
You can now create experimental protocols more quickly by using templates and macros. Roche lightcycler 480 realtime pcr run setup instructions. Optimized for the platebased roche lightcycler 480. It seems that this file holds some kind of experiment data. Pcr primer and probe design software used with all lightcycler carousel based instruments and lightcycler 480 instruments. Roche diagnostics 04729749001 lightcycler 480 multiwell plate 384, white 5 x 10 plates includes sealing foils. Start the lightcycler 480 software by doubleclicking the icon. The cycler has 4 detection channels, which detect hydrolysis 5nuclease taqman probes either as single color or multiplex samples. This compact benchtop instrument uses a fully integrated multi well plate that can be switched out for either 96 or 384well plate formats without recalibration. Service manual light cycler please note that this is only a. The lightcycler 96 is a realtime pcr cycler for medium sample throughput. The lightcycler 480 system is a proven highperformance, mediumto highthroughput pcr platform that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation.
The combination of both components allows complex applications such as product analysis, quantification and mutation analysis. We still have a 12yearold lightcycler from idaho technology, before roche bought this company. Programming the lightcycler 480 sybr green i protocol, v. Warranty information on warranty conditions are specified in the sales. The cycler has 4 detection channels, which detect hydrolysis 5nuclease.
The capillary based system works with hot and cold air allowing rapid ramping 30 cycles can be performed within 20 minutes. Roche lightcycler 96 biotecon diagnostics biotecon. Lightcycler 1536 real time pcr system roche diagnotic. Has anybody used the light cycler 480 system by roche. M of each of the probes, an appropriate amount of mastermix, and 0. Enter the username and assigned password to log in to the lightcycler 480 software. The lightcycler instrument of roche molecular biochemicals, mannheim, ge rmany, is a thermocycler for the rapid analysis of pcr applications. The software is compatible with the regulations for electronic records subpart.
It integrates all software modules required to perform and analyze any type of experiment using the lightcycler 2. Roche molecular diagnostics has introduced the lightcycler instruments, the fastest thermocyclers with online monitoring of pcr based on fluorescence detection. Forge ahead into a new era in highthroughput realtime pcr with the new lightcycler 1536 instrument. The amplification of a viral target in twofold dilutions ranging from 1x10 2 to 1x10 5 copies per reaction is shown. Roche has brought many highly effective drugs onto the market and is a world leader in innovative cancer drugs. The lightcycler 480 instrument ii 96 to 384well throughput, versatile for experienced users the lightcycler 480 instrument is a highperformance, flexible throughput qpcr platform with interchangeable 96 or 384well blocks that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and. Other areas include viral infections, metabolic, central nervous system disorders. Flexibly switch between plate formats and thermal block cyclers in just a few minutes with no recalibration time, and benefit from additional userfriendly software. The lightcycler 480 ii is a realtime pcr cycler for mid to high sample throughput. Realtime ready custom panels create your specific gene expression panel with realtime ready assays of your choice on lightcycler 480 multiwell plates 96 or 384, using the realtime ready configurator. Combining the rigidity of a polycarbonate frame with thin walled polypropylene wells, the armadillo plate provides superior thermal cycling performance under all con.
The lightcycler 480 system offers high resolution meltingbased gene scanning as an integrated solution on a platebased realtime pcr instrument. Draggable legend and ability to not display it or on several columns. Precisely engineered for miniaturization and parallelization, this latest innovation. The system features the lightcycler 480 instrument, a versatile, platebased realtime pcr device that supports mono or multicolor applications. Lightcycler technology is the most innovative and rapid possibility for carrying out and simultaneously evaluating pcr experiments. It offers fast and accurate analysis that impresses with its intuitive and userfriendly software. The lightcycler 480 system is a highperformance, medium to highthroughput pcr platform 96 or 384well plates that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation.
Roche life science realtime ready overview lightcycler. The lightcycler 480 ii with led is suitable for detection systems with different probe types such as hybridization probes or hydrolysis 5nuclease taqman probes. Armadillo pcr plate, 96well, clear, semiskirted, low. We may use the information that you provide to us to take the steps necessary to respond to your request, for example, you may submit a medical information request, inquire about a product, or subscribe to one of our mailing lists. The lightcycler 480 ii with led is suitable for detection systems with. In preparation for real time polymerase chain reaction applications, small segments of dna or rna are isolated and placed in pcr plates. Under the measurement tab found on top left, click on the pencil icon in the center of the screen to select the channel of choice and the dye of choice within the channel. Roche lightcycler 480 ii shared resources fred hutchinson. Realtime ready assays are available as single assays for the selected target or preplated on lightcycler 480 multiwell plates 96 or 384. Discover the new lightcycler 96 realtime pcr system a compact and smart device thats the perfect companion for qpcr newbies and experts. New experiment based on roche template for a new empty experiment. Lightcycler 480 software analysis screen for absolute quantification.
433 1413 217 934 703 1354 287 397 1127 1291 134 105 530 201 1474 761 1039 211 601 1131 594 1454 1159 255 1376 79 1105 378 653 1060 156 692 623