M protein molecular mass marker pageruler unstained protein ladder fermentas. Pageruler prestained protein ladder thermo fisher scientific. The thermo scientific pageruler prestained nir protein ladder is a mixture of 10proteins ranging from 11kda to 250kda, which produces 10 welldefined blue bands by sdspage see website for product images. Biotinylated protein ladder detection pack is designed to detect the molecular weight ladders on western blots when using the horseradish peroxidase hrp based western detection system. The pink plus prestained protein ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency. Pageruler prestained protein ladder is a mixture of ten blue, orange, and greenstained recombinant proteins 10 to 170 kda for use as size standards in protein electrophoresis sdspage and western blotting. The hxstable prestained protein ladder is a threecolor protein standard with 12 prestained proteins covering a wide range molecular weights from 10 to 245 kda.
The protein ladder is conveniently packaged and ready to. Pageruler prestained protein ladder 10 170 kda migration patterns were determined using respective nupage precast gels. Color prestained protein standard, broad range 10250 kda. For more about pageruler prestained protein ladder concentration, please subscribe to our website newsletter now. Prestained protein ladder, broad range 10230 kda is a mixture of highly purified recombinant proteins covalently coupled to a blue dye that resolves into 12 sharp bands when electrophoresed.
Precision plus protein prestained standards are available in dual color, all blue, kaleidoscope, and dual xtra options. Each marker is covalently bound to a pink or blue colour chromaphore to produce a ladder of evenly. Shop online for a wide selection of thermo scientific pageruler plus prestained protein ladder, 10 to 250 kda mixture of 9 blue, orange and. The ladder is visualized by sdspage using coomassie or silver stains or detected in western blots with protein stains. For easy reference, the 50 kda protein band has a greater intensity than the other proteins in the ladder. Resolving and identifying protein of interest using.
Pageruler prestained protein ladder, 10 to 180kda sdspage. Instructions pageruler prestained nir protein ladder. Accuruler plus prestained protein ladder lamda biotech. Prestained protein ladders and unstained protein ladders abcam. The prestained protein marker triple color, edta free, 10250 kda is a prestained mixture of ten recombinant proteins ranging from 10 kda to 250 kda.
Features of the benchmark prestained protein ladder. Proteins are covalently coupled with a blue chromophore except for two reference bands one. Thermo scientific pageruler plus prestained protein ladder is a mixture of nine blue, orange, and greenstained proteins 10 to 250 kda for use as size standards in protein electrophoresis sdspage and western blotting. Covalently coupled dyes allows easy visualization of the ladder during, and after electrophoresis. Pageruler plus prestained protein ladder, 10 to 250 kda. Fig 7 pageruler prestained protein ladder thermo scientific ltd page 12 iii from bioc 46 at the university of hong kong. This reagent must be used in combination with cruz marker mw tag detection antibodies available only from santa cruz biotechnology, inc. Pageruler prestained protein ladder 2 x 250 l from thermo. Description this prestained protein mw marker is designed for monitoring the progress of sdspolyacrylamide gel electrophoresis, for assessing transfer efficiency onto pvdf, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or western blot detection reagents. Thermo scientific pageruler unstained broad range protein. M protein molecular mass marker pageruler unstained. However, an unstained protein ladder can only be visualized following staining with coomassie, or a similar nonspecific protein stain. Prestained protein ladder broad molecular weight 10245 kda.
Thermo scientific pageruler prestained protein ladders the thermo scientific pageruler and pageruler plus prestained protein ladders are comprised of highly purified recombinant proteins bound to three different chromophores for easy protein. We checked neural protein expression using this protein ladder as a marker for proteins of various molecular weights. The apparent molecular weights of the colorplus prestained. Prestained protein ladders and unstained protein ladders. Mix thoroughly to ensure the solution is homogeneous. Thermo scientific pageruler prestained protein ladder is a mixture of 10 blue, orange, and greenstained proteins 10 to 180 kda for use as size standards in protein electrophoresis sdspage and western blotting. The pack contains biotinylated protein ladder and antibiotin, hrplinked antibody. Page 3 example the apparent molecular weights of the protein bands in benchmark prestained protein ladder are shown below. Product information thermo scientific pageruler plus. M protein molecular mass marker pageruler unstained protein ladder fermentas from biology 402 at peking university.
Page 4 benchmark prestained protein ladder limited use label. Color prestained protein standard, broad range is a mixture of highly purified, prestained proteins, covalently coupled with a blue chromophore, and two reference bands one orange and one green at 80 kda and 25 kda, respectively, that resolves into 12 sharp bands when electrophoresed. Prestained markers are brighter for visual robustness throughout western blotting to accurately estimate mw, monitor electrophoresis and transfer efficiency. The apparent molecular weight of each protein kda has been determined by calibration against an unstained protein ladder in each electrophoresis conditions. The accuruler plus prestained protein ladder contains 12 prestained proteins, covering a wide range of molecular weights from 10 to 245kda. Thermo scientific pageruler plus prestained protein ladder is a mixture of nine 9 blue, orange and greenstained proteins 10 to 250kda for use as size standards in protein electrophoresis sdspage and western blotting. The apparent molecular weight of the prestained proteins are given in the table below. Pdf bulletin 2998 download the protein standards application guide. With, one of our best sellers, 3 tricolored prestained protein marker, you can load up over 300 wells for western blotting application.
Mxstable prestained protein ladder dna protein ladders. Procedure from instruction manual for biorad transblot sd semidry electrophoretic transfer cell. Pink plus prestained protein ladder 10175kda cleaver. Bluerayprestainedproteinmarker 10180kda readytouseprestainedproteinladder cat. Unstained protein ladders are more accurate for sizing proteins, as the dyes used in prestained ladders can slightly distort the apparent size of the protein ladder proteins on the gel. Product information thermo scientific pageruler unstained. Prestained protein marker triple color, edta free, 10250. Prestained protein ladder v is a threecolor protein standard with 10 prestained proteins covering a wide range of molecular weights for 10 to 180 kda. Thermo scientific pageruler prestained protein ladders the thermo scientific pageruler and pageruler plus prestained protein ladders are comprised of highly purified recombinant proteins bound to three different chromophores for easy protein molecular weight determination. Benchmark prestained protein ladder allows you to accurately determine molecular weight and evaluate. Coloured reference bands serve as visual indicators of electrophoresis run progression and the efficiency of western transfer onto membranes.
Prestained protein marker, broad range 11190 kda is a mixture of purified proteins covalently coupled to a blue dye that resolve to a series of 11 bands between 11 and 190 kda following electrophoresis. The readytouse bluestain protein ladder covers a molecular weight between 24510 kda, and is used for protein electrophoresis operations. Load the following volumes of the ladder on sdspage gel. It is prestained, multicoloured peptides makes eassy to identify the protein of interst bands. It is prestained and has multicoloured peptides which makes it easy to identify. In lowpercentage gels prestained protein ladder contains 11 proteins that resolve into sharp, tight bands in the range of 10175 kda. Get to know more about ketogenic diet and pageruler prestained protein ladder concentration here on this site. Broad range prestained protein marker3245 kda pl00002. Two orange reference bands at 70 kda and 25 kda and one green reference band at 10 kda highlight the bluestained protein ladder. The benchmark prestained protein ladder is designed for use in sdspage using trisglycine gels for estimation of protein molecular weight, and evaluation of western transfer efficiency. And to give sm6071 answer on the fermentas pageruler protein ladder sm ops question, i would agree that its then more specific fractionation techniques the fermfntas your workouts. Unstained versus prestained protein ladders scientist solutions. Five microliters of benchmark prestained protein ladder was separated on a.
Store the protein ladder for up to three months at 4c or for one year at 20c. Prestained protein marker, broad range 7175 kda neb. Applications of precision plus protein prestained standards. Mxstable prestained protein ladder is a threecolor protein standard with 10 prestained proteins covering a wide range molecular weights for 10 to 180 kda. The molecular weights of the proteins have a lottolot variation of approximately 5%. Bluestain protein ladder, 11245 kda goldbio asset 1. Acuteband prestained protein ladder save money for your lab. Prestained protein ladder, broad range 10230 kda neb. Datasheet for colorplus prestained protein ladder, broad. Proteins are covalently coupled with a blue chromophore except for two reference bands one green and one red band when. Each marker is covalently bound to a pink or blue colour chromaphore to produce a ladder of evenly interspersed bands of uniform intensity. The blueray prestained protein marker is a threecolor protein. Proteins are used in routine laboratory procedures such as binding enzymes or coupling peptides to carrier proteins.
It is designed for monitoring protein separated during polyacrylamide gel electrophoresis, verification of western transfer efficiency on membranes and for sizing of proteins. Test our acuteband prestained protein ladder mw range 6. Proteins are covalently coupled with a blue chromophore except for two reference bands one green. Thermo scientific pierce protein molecular weight marker. The thermo scientific pageruler plus prestained protein ladders are a mixture of nine recombinant proteins ranging from 10 kda to 250 kda. Cst prestained protein marker, broad range 11190 kda. Colorplus prestained protein ladder, broad range 10230 kda. Thaw the ladder either at room temperature or at 3740c for a few minutes to dissolve precipitated solids. Thermo scientific pageruler plus prestained protein ladder, 10 to 250 kda mixture of 9 blue, orange and greenstained proteins 10 to 250kda used as size standards in protein electrophoresis sdspage and western blotting. The apparent molecular weights of the colorplus prestained protein ladder bands were determined on invitrogen novex 1020% trisglycine sdspage gels 1. Does pierce pageruler prestained protein ladder a mix of.
Monitor protein migrationsample in sdspage, monitor protein transfer onto membranes during western blotting, sizing of proteins on sdspage and western blots introduction prestained protein ladder v is a threecolor protein standard with 10 prestained proteins covering a wide range of molecular weights for 10 to 180 kda. Thermo scientific pageruler prestained protein ladder is a mixture of ten 10 blue, orange and greenstained proteins 10 to 180 kda for use as size standards in protein electrophoresis sdspage and western blotting. The pageruler unstained broad range protein ladder is supplied readytouse. Home life science research biorad products electrophoresis and blotting protein electrophoresis and western blotting protein standards and ladders molecular weight markers recombinant protein standards. So, does pierce pageruler prestained protein ladder a mix of phosphorylated protein. Opal prestained protein standard 10180kda mb2090500. Blue prestained protein standard, broad range 11250 kda.
The ladder consists of six bands which appear on the final western blot image. Product information thermo scientific pageruler unstained protein. In addition, the ladder proteins except for the 5kda peptide contain an integral streptag ii sequence and may be detected on western blots using streptactin conjugates. It is prestained and has multicoloured peptides which makes it easy to identify the band of the protein of interest. The blueray prestained protein marker is a threecolor protein standard with 10 prestained purified proteins. The acuteband prestained protein ladder is a three color protein standard with 10. Hxstable prestained protein ladder dna protein ladders.
Proteins are covalently coupled with a blue chromophore except for two reference bands, one green at 25 kda and one red one at 75 kda respectively, when separated on sdspage trisglycine buffer. Pageruler plus prestained protein ladders are a mixture of nine blue, orange and greenstained proteins 10 to 250 kda for use as size standards in protein electrophoresis sdspage and western blotting. Download a pdf containing pricing for our full product list. Unstained protein ladder is a mixture of 12 recombinant, highly purified proteins, which resolve into clearly identifiable sharp bands from 10250 kda when analyzed by sdspage and stained with coomassie brilliant blue r250 1,2. Three different chromophores are bound to the proteins, 9 bands with blue, one red at 70 kda and one green at 25 kda.
Briteruler prestained protein ladder 9306 biovision. Pink plus prestained protein ladder 10175kda quantity. Blueray prestained protein marker, prestained protein mw. Prestained protien ladder molecular biology proteomics. The accuruler prestained protein ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kda.
Thermo scientific pageruler plus prestained protein. Pageruler prestained protein ladder pageruler plus prestained protein ladder prestained protein molecular weight marker unstained protein 816% trisglycine sdspage gel blot gel blot protein. Benchmark prestained protein ladder contains 10 proteins ranging in apparent molecular weight from 6 to 180 kda for use in monitoring electrophoresis or. Ladder is a prestained mixture of ten recombinant proteins ranging from 10 kda to 180 kda. Place your order before 8pm est for overnight delivery. Mixes very good with about why the right fermentas pageruler protein ladder sm eating plan works list in its category for more than a year. Prestained protein standards life science research biorad. Protein ladder, protein gel electrophoresis size marker, bluegreenred protein marker. Unstained protein ladder, broad range 10250 kda neb.
Biovisions briteruler prestained protein ladder is a readytouse formulation which does not require boiling, contains 10 prestained proteins covering a wide range of molecular weights 10180 kda and includes a red reference band at 75 kda and a green reference band at 25 kda. Benchmark prestained protein ladder thermo fisher scientific. Precision plus protein kaleidoscope prestained protein. Opal prestained protein standard 10180kda is designed for monitoring protein separated during sdspolyacrylamide gel electrophoresis, verification of western blot transfer efficiency on membranes pvdf, nylon, or nitrocellulose and for approximate sizing of proteins. The protein ladder is supplied in a readytouse format for direct loading. Blueelf prestained protein marker, prestained protein mw marker. Prestained protein ladders can have a sligtly different mobility and mass than the same peotein ladder which is not prestained.
The thermo scientific pageruler prestained protein. Benchmark prestained protein ladder must show 10 major bands when separated by electrophoresis on a novex 420% trisglycine gel. Color prestained protein standard, broad range 11245 kda neb. Under suggested conditions, blultra prestained protein ladder resolves 10 major bands in 412% bistris gelmes buffer and after western blotting to the nitrocellulose membrane. Thermo scientific pageruler plus prestained protein ladder. The blue prestained protein standard, broad range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, that resolves into 11 sharp bands when electrophoresed. Pageruler prestained protein ladder, 10 to 180 kda. The color prestained protein standard, broad range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, and two reference bands one orange and one green at 72 kda and 26 kda, respectively, that resolves into 11 sharp bands when electrophoresed. The protein concentrations are carefully balanced for even intensity. Prestained protein ladder is designed for monitoring protein separation during sdspolyacrylamide gel electrophoresis, verification of western transfer efficiency on membranes pvdf, nylon or nitrocellulose and for approximating the size of proteins.
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